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纯度

> 90 % as determined by SDS-PAGE.

内毒素

< 1.0 EU per μg protein as determined by the LAL method.

生物活性

The 2019-nCoV Spike Protein (S1 Subunit, mFc Tag) can bind with Human ACE2 in ELISA assay.

蛋白构建：

A DNA sequence encoding the NCP-CoV(2019-nCoV) spike protein S1 Subunit was expressed with the Fc region of mouse IgG1 at the C-terminus.

表达宿主：

HEK293 Cells

种属

2019-nCoV

预测 N 端

Val

S1蛋白质氨基酸序列：

VNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYE

Mouse IgG1 Fc Tag Seq:

         10         20         30         40         50         60
 PRDCGCKPCI CTVPEVSSVF IFPPKPKDVL TITLTPKVTC VVVDISKDDP EVQFSWFVDD
 
         70         80         90        100        110        120
 VEVHTAQTQP REEQFNSTFR SVSELPIMHQ DWLNGKEFKC RVNSAAFPAP IEKTISKTKG
 
        130        140        150        160        170        180
 RPKAPQVYTI PPPKEQMAKD KVSLTCMITD FFPEDITVEW QWNGQPAENY KNTQPIMDTD
 
        190        200        210        220
 GSYFVYSKLN VQKSNWEAGN TFTCSVLHEG LHNHHTEKSL SHSPGK

分子量：

The recombinant NCP-CoV(2019-nCoV) Spike Protein (S1 Subunit, His Tag) consists of 877 amino acids and predicts a molecular mass of 98.35 kDa.

缓冲液

Lyophilized from sterile PBS, pH 7.4.

运输方式

proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.