关键词:抗体制备抗体标记重组蛋白表达

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2019新型冠状病毒S蛋白(RBD,Fc标签


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产品品牌:Frdbio 产品货号:nCoV-S
保存温度:-20 有效日期:0个月
英文名称:2019-nCoV Spike Protein (RBD, Fc Tag)
规      格: 100ug 20mg
价      格:优惠价 ¥4200
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Method of Production  : Transient transfection in 293 cell culture    

Method of Purification  : Protein A affinity chromatography and SEC

Formulation:   PBS pH7.4

Concentration:   2.50 mg/mL

Eedotoxin1EU/ug

0.2μm FiltrationYes

表达宿主:Human Cells

纯度:> 95 % as determined by SDS-PAGE.

内毒素:<1.0 EU per μg protein as determined by the LAL method.

蛋白构建:

A DNA sequence encoding the receptor binding domain (RBD,319-541aa) of 2019-nCoV spike was expressed with the Fc region of mouse IgG1 at the C-terminus.

GIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTN

融合Fc标签氨基酸序列:

PRDCGCKPCICTVPEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVEVHTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTISKTKGRPKAPQV

YTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMDTDGSYFVYSKLNVQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPGK

分子量:

The recombinant receptor binding domain (RBD) of 2019-nCoV spike consists of 457 amino acids and predicts a molecular mass of 51.5 kDa.

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