PRODUCT SPECIFICATIONS

Product Name: SP2/0 .cell

Cat No. :MAC0022

Tissue: Spleen

Cell Type Hybridoma: B lymphocyte

Product Format  culture: cell

Morphology: Lymphoblast-like

Culture Properties:   Suspension

Biosafety Level:  1

Strain:BALB/c

Applications: This cell line is a fusion partner for production of somatic cell hybrids.

Complete Growth Medium:    The base medium for this cell line is DMEM(Frdbio, Catalog No. MAC0017). To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing:     Cultures can be maintained by the addition of fresh medium or replacement of medium. Maintain cell density between 5 X 104and 5 X 105 viable cells/mL. Some cells can attach and be transferred by shaking them loose.

Medium Renewal: Add fresh medium every 2 to 4 days (depending on cell density)

Cryopreservation:Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions Temperature: 37°C

*本试剂仅供实验室研究使用