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Recombinant Streptavidin(Core),r-cSA


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产品品牌:Frdbio 产品货号:SAB0011
保存温度:-20℃ 有效日期:0个月
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PRODUCT SPECIFICATIONS

Product Name: Recombinant StreptavidinCore),r-cSA

Introduction:Streptavidin (SA) is a homotetramer protein secreted by Streptomyces avidinii during its growth. One mole of streptavidin can bind to four moles of biotin. Because SA does not contain glycosyl, it has a lower non-specific binding than avidin in detection applications. It has been widely used in enzyme-linked immunosorbent assay(ELISA), immunohistochemistry, time-resolved immunofluorescence (TRFIA), quantitative PCR, single strand DNA preparation, biological purification, monoclonal antibody preparation and other biotechnology fields. Compared with natural streptavidin, recombinant core streptavidin can remove the non activity related sequences and retain only the activity related core sequences. The stability and solubility of recombinant core streptavidin are better than natural SA.

Cat No. : SAB0011

Product form: Lyophilized powder. Before lyophilization, the solution was 5 mM PB (4 mM Na2HPO4, 1 mM NaH2PO4, pH 7.4)

Molecule Weight13.3 kDa (monomer), 53.2 kDa ( tetramer).

Isoelectric point 6.09

Theoretical activity 18.3 U/mg SA (r-cSA:Biotin = 1:4(mol:mol))


Actual activity ≥15.1 U/mg SAimproved Green's function method

Purity ≥95%SDS-PAGE

A280nm (1 mg/mL):3.16

Endotoxin ≤4 EU/mg

Source Escherichia coli

Preservation conditions -20℃

Term of validity 36 months

 

Note: This product is for research use only,not for diagnostic or therapeutic use.


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