ED50 < 0.25 ng/mL, measured by the cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 4 × 106 units/mg.
17.1 kDa, observed by reducing SDS-PAGE.
> 95% by SDS-PAGE analysis.
Lyophilized after extensive dialysis against PBS.
Reconstituted in ddH2O at 100 μg/mL.
< 0.2 EU/μg, determined by LAL method.
Lyophilized recombinant remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhFGF-basic remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFLRIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANRYLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYTSWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS